Gene description for CD247
Gene name	CD247 molecule
Gene symbol	CD247
Other names/aliases	CD3-ZETA CD3H CD3Q CD3Z CD3ZETA IMD25 T3Z TCRZ
Species	Homo sapiens
Experiment description of studies that identified CD247 in T cells
1 Experiment ID 17 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting Immunoelectron Microscopy PubMed ID 11907077    Organism Homo sapiens Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex. Authors Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C Journal name JIMMU Publication year 2002 Sample T cells Sample name Jurkat T cell blasts E+ cells MART-1+ T cell Isolation/purification methods Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Immunoelectron microscopy EV-TRACK -
2 Experiment ID 18 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method FACS PubMed ID 11907077    Organism Homo sapiens Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex. Authors Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C Journal name JIMMU Publication year 2002 Sample T cells Sample name Jurkat T cell blasts Isolation/purification methods Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study FACS EV-TRACK -
3 Experiment ID 1004 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23463506    Organism Homo sapiens Experiment description The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. Authors Perez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó Journal name J Biol Chem. Publication year 2013 Sample T cells Sample name T lymphoblasts - exosomes pulldown with CD81 as a bait Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV130110: EV-METRIC:22%
4 Experiment ID 1008 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 23463506    Organism Homo sapiens Experiment description The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes. Authors Perez-Hernandez D, Gutiéez-Váuez C, Jorge I, Ló-MartíS, Ursa A, Sáhez-Madrid F, Váuez J, Yáz-Mó Journal name J Biol Chem. Publication year 2013 Sample T cells Sample name T lymphoblasts Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130110: EV-METRIC:22%